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Antimicrobial Effect of Nano-Zinc Oxide and Nano-Chitosan Particles in Dental Composite Used in Orthodontics

Authors: AmirHossein Mirhashemi, Abbas Bahador, MohammadZaman Kassaee, Ghazaleh Daryakenari, MohammadSadegh Ahmad-Akhoundi, Ahmad Sodagar
Keywords: Chitosan, Zinc Oxide, Orthodontics
Journal of Medical Bacteriology, Vol.2, No.3-4, 2013,Page:1-10

BackgroundIncidence of  white  spots due  to  demineralization of  enamel  and  gingival problems  is  an  unacceptable  result  of  orthodontic  treatment.  Plaque  accumulation  and bacterial biofilm growth are responsible for these phenomena. The resin-based dental composites used as bonding agents in orthodontics play a major role in mentioned problems. As recent researches assert the antimicrobial effects of chitosan (CS) and zinc oxide (ZnO) nanoparticles  (NPs),  it  seems  that  adding  these  nanoparticles  to  the  composite  can  be beneficial in reducing the number and function of microorganisms. The aim of this study was to  evaluate  the  antimicrobial  effects  of  ZnO-NP  and  CS-NP-containing  orthodontic composite.

MethodsAntibacterial effectiveness of ZnO-NPs and CS-NPs was assessed in four groups against Streptococcus mutansStreptococcus sanguis and Lactobacillus acidophilus grown both planktonic and as a biofilm on composites. One group as the unmodified control group and  three  groups  consisting  of  three  different  concentrations of  ZnO-NPs  and  CS-NPs mixture: 1%, 5% and 10% (1:1 w/w). 108 CFU/ml microorganism suspensions were provided with spectrophotometer. Biofilm formation was quantified by viable counts. Disc agar diffusion (DAD) test was carri