Tehran University of Medical Sciences
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Ghasemali Khorasani

In vitro and in vivo investigation of a novel amniotic-based chitosan dressing for wound healing


Authors: Maryam Momeni, Mohammad Zarehaghighi, Mirhamed Hajimiri, Ghasemali Khorasani, Rassoul Dinarvand, Abdolhossein Nekookar, Niloofar Sodeifi, Pardis Khosravani, Niloofar Shayanasl, Marzieh Ebrahimi
wound repair and regeneration, Vol.26, No.1, 2018,Page:87-101

ABSTRACT
It is more than a decade that amniotic membrane has been used as a wound
dressing because of its anti-inflammatory, anti-microbial, anti-fibrotic, antiscarring
properties, as well as its pain relieving and epithelialization promoting
features. However, amniotic membrane had limited applications because it needs
to suture in surgery, is highly fragile, firmly adhere to the wound and may cause
bleeding and pain when changing the bandage. This study investigated the
possibility of development of a novel amniotic-based chitosan gel dressing as a
potential wound repair substrate with marked efficacy. In this experiment,
amniotic gel prepared based on chitosan/PVP gel containing human amniotic
membrane extract (AME-Gel) was investigated in terms of wound-healing
efficacy and scar preventive effects in a rat burn model. The levels of reepithelialization
and dermal regeneration were examined by histological
assessment using H&E and Masson’s trichrome staining. Also, we clarified the
mechanism of healing and cytokine-releasing activities of AME as well as its
effect on epithelization, angiogenesis, and fibroblast growth and migration. Our
results revealed that AME-Gel induces epidermal and dermal regeneration at a
shorter time through formation of granulation tissue, enhancement of fibroblast
proliferation, and improvement of blood capillary formation concomitant with
developing collagen bundles. Therefore, AME-Gel could be considered a simple
and easy to be used as a biological dressing for any type of superficial burn
wounds, without any adverse effects.